Larval RNA Interference in the Red Flour Beetle, <em>Tribolium castaneum</em>
نویسندگان
چکیده
منابع مشابه
Larval RNA Interference in the Red Flour Beetle, Tribolium castaneum
The red flour beetle, Tribolium castaneum, offers a repertoire of experimental tools for genetic and developmental studies, including a fully annotated genome sequence, transposon-based transgenesis, and effective RNA interference (RNAi). Among these advantages, RNAi-based gene knockdown techniques are at the core of Tribolium research. T. castaneum show a robust systemic RNAi response, making ...
متن کاملDissecting Systemic RNA Interference in the Red Flour Beetle Tribolium castaneum: Parameters Affecting the Efficiency of RNAi
The phenomenon of RNAi, in which the introduction of dsRNA into a cell triggers the destruction of the corresponding mRNA resulting in a gene silencing effect, is conserved across a wide array of plant and animal phyla. However, the mechanism by which the dsRNA enters a cell, allowing the RNAi effect to occur throughout a multicellular organism (systemic RNAi), has only been studied extensively...
متن کاملDoublesex target genes in the red flour beetle, Tribolium castaneum
Sex determination cascade in insects terminates with the production of sex-specific protein, Doublesex (Dsx). We identified the dsx homolog (Tcdsx) in Tribolium castaneum. The pre-mRNA of Tcdsx is sex-specifically spliced into three female (Tcdsxf1, Tcdsxf2 and Tcdsxf3) and one male-specific (Tcdsxm) isoforms. Cis-regulatory elements potentially involved in sex-specific splicing of the Tcdsx pr...
متن کاملRationale to sequence the genome of the red flour beetle,
Overview: In a world of worthy candidates, there are several compelling reasons to sequence the genome of the red flour beetle Tribolium castaneum. First and foremost, Tribolium is one of the most sophisticated genetic model organisms among all higher eukaryotes. Among arthropods, only Drosophila offers greater power and flexibility of genetic manipulation. Second, the Tribolium genome sequence...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2014
ISSN: 1940-087X
DOI: 10.3791/52059